Measuring and quantifying molecular interactions are increasing demands in the biological community in response to the explosion of information coming from the application of highthroughput methods to various problems. The opening of the field of biophysical measurements to new users creates the need for basic and advanced training courses covering fundamental knowledge of the instrumental methods, practice in setting-up and performing experiments, and data analysis and interpretation.
The first day is a minisymposium with lectures on the main methods (see below) and on related techniques such as dynamic light scattering (DLS), microscale thermophoresis (MST), differential scanning fluorimetry (DSF) and fluorescence spectroscopy techniques, small-angle scattering, native mass spectrometry (MS), cryo electron microscopy (Cryo-EM), super resolution microscopy, NMR. It will be followed by platforms visits and instrument demos by the tutors and the sponsors. The participants will have to select 3 or 4 visits/demos among the proposed visits/demos.
Then, during four days, the course will provide theoretical introduction, practicalities to prepare samples and set up experiments, and basic and advanced data analysis in several modern methods for monitoring and quantifying molecular interactions: analytical ultracentrifugation (AUC), isothermal titration calorimetry (ITC), surface plasmon resonance (SPR) or biolayer interferometry (BLI).
The workshop will focus on hands-on data analysis, using both tutorials with sample data and real data analysis (including one-on-one sessions with course tutors). The course will put emphasis on the complementarity of these different methods in the field of macromolecular biology and on the combined analysis of datasets coming from different methods. Practical data analysis will be demonstrated using the computational tools SEDFIT, SEDPHAT, GUSSI, EVILFIT and NITPIC.
The course will take place at the Institut de Biologie Structurale (IBS) , using the Integrated Structural Biology, Grenoble (ISBG) core facilities of the Partenariat for Structural Biology (PSB) on the EPN campus.
30 October 2019
30 October 2019